Impact of region-of-interest size and location on quantitative contrast-enhanced ultrasound of canine splenic perfusion

Abstract Background During contrast enhanced ultrasound (CEUS), the features of regions interest (ROI) can affect value perfusion-related parameters obtained from a time intensity curve (TIC). In veterinary medicine, conflicting have been reported on influence ROI size and location renal CEUS. There are some disagreeing evidences regarding optimal method for selecting in quantitative analysis perfusion using The aim this study was to evaluate effect ROIs spleen conscious dogs variables determined sulphur hexafluoride contrast-enhanced ultrasounds. Results A prospective observational 15 client-owned mixed-breed adult performed system equipped with contrast-tuned imaging technology. Qualitative assessments enhancement pattern were carried out. Three square (0.05 cm 2 ) manually drawn row spaced 1 mm apart, placing adjacent at three different depths. medium rectangular (0.3 include 3 smallest each row, indicated by letters A, B C, single large (1 containing all previous ROIs. Software time-intensity curves generated within allowed us calculate parameters: peak enhancement, peak, regional blood flow, mean transit volume. coefficient variation blood-related always lower larger than other smaller B, positioned proximally medially, levels respectively, showed similar coefficients largest ROI. variance model exhibited significant canine perfusion, reduction distal Conclusions recommendation CEUS examination dog’s is analyze splenic drawing sufficiently proximal beam parenchyma. This may be clinical relevance diagnosis diseases.